In this study, a sensitive, simple, and reliable HPLC method for quantification of pregabalin in human plasma was developed and\nvalidated. 1-Fluoro-2,4-dinitrobenzene was used as precolumn derivatization agent. For chromatography, an analytical reversed\nphase (C18) column and a mixture of Na2HPO4 10mM(pH 8.0)ââ?¬â?methanol (35 : 65 v/v) were used as stationary and mobile phase,\nrespectively. Detection was performed using a UV detector tuned at 360 nm. The linearity of the method was tested over the\nconcentration range 1ââ?¬â??4500 ng/mL in 500 ????L of human plasma and satisfactory results were obtained (r2 > 0.999). The method\nshowed good precision and accuracy in terms of withinââ?¬â?between days relative standard deviations and percent deviation from\nnominated values (in the range of 4.3ââ?¬â??12.7% and 2.6ââ?¬â??8.0%, resp.).Thelimit of quantification of the method was found to be 1 ng/mL\nwhich is better than previously reported method and indicates its potential application for sensitive bioanalysis
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